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              高效液相色譜和毛細管電泳食品中苯甲酸鈉含量的測定

              時間:2019-07-20 23:00來源:畢業論文
              實驗采用高效液相色譜法和毛細管電泳法研究了可樂、雪碧中防腐劑苯甲酸鈉的含量,并分別建立了高效液相色譜和毛細管電泳對可樂、雪碧中苯甲酸鈉含量的分析測定方法

              摘要:食品中苯甲酸鈉含量的測定,無論對食品防腐劑使用范圍,還是食品安全都具有非常重要的意義。實驗采用高效液相色譜法和毛細管電泳法研究了可樂、雪碧中防腐劑苯甲酸鈉的含量,并分別建立了高效液相色譜和毛細管電泳對可樂、雪碧中苯甲酸鈉含量的分析測定方法。所建立的高效液相色譜測定方法對苯甲酸鈉在0.01-0.10mg/ml范圍內線性關系良好,精密度和重復性的相對標準偏差均小于2%,加標回收率在5%偏差范圍內,可用于可樂、雪碧中苯甲酸鈉含量的研究。而毛細管電泳測定方法對苯甲酸鈉在0.01-0.20mg/ml范圍內線性關系良好,精密度和重復性的相對標準偏差均小于10%。高效液相色譜法測定結果表明,可樂、雪碧中苯甲酸鈉的含量均達到了0.18mg/ml,毛細管電泳法測定結果表明雪碧中其含量為0.16mg/ml。以上結果為可樂、雪碧中苯甲酸鈉含量及食品研究提供了依據。37304
              畢業論文關鍵詞:苯甲酸鈉;可樂、雪碧;含量測定;高效液相色譜法;毛細管電泳法
              Determination of Sodium Benzoate Content in Foods
              Abstract: The determination of sodium benzoate content in foods has a very important significance to both the scope of the use of preservatives and the food safety. This experiment use high performance liquid chromatography method (HPLC) and capillary electrophoresis method (CE) to research the content of sodium benzoate in coca cola and sprite. In this experiment, we established the high performance liquid chromatography method (HPLC) and capillary electrophoresis method (CE) analysis and determination method for the content of sodium benzoate in coca cola and sprite respectively. The HPLC method we established has a good linear relation in the range of 0.01-0.10mg/ml, both the relative standard deviation of precision and reproducibility are less than 2%, the recovery of standard addition is less than 5% among 0.01-0.10mg/ml, which could be used in the determination of sodium benzoate content in coca cola and sprite. However,The CE method we established has a good linear relation in the range of 0.01-0.20mg/ml, both the relative standard deviation of precision and reproducibility are less than 10% among 0.05-0.20ml。The result of high performance liquid chromatography (HPLC) shows that content of sodium benzoate content in coca cola and sprite both reached to 0.18mg/ml, and the result of capillary electrophoresis method(CE) shows it is 0.16mg/ml in sprite . The results provide the basis to the content of sodium benzoate in coca cola and sprite and food safety.
              源`自^六;維'論.文;網www.kj9998.cn

              Key Words: sodium benzoate; coca cola and sprite; content determination; high performance liquid chromatography method (HPLC); capillary electrophoresis method (CE)
              目錄
              1 引言    1
              1.1 論文的目的和意義    1
              1.2 國內外研究現狀、水平和發展趨勢    2
              1.2.1 苯甲酸鈉與食品防腐劑相關性    2
              1.2.2 苯甲酸鈉與食品安全的關系    2
              1.2.3 苯甲酸鈉含量的研究方法    3
              1.2.4 苯甲酸鈉的研究概況    3
              1.3 本論文擬解決的問題    4
              2 高效液相色譜法對可樂、雪碧中苯甲酸鈉含量的測定    5
              2.1 概述    5
              2.2 儀器和試劑    6
              2.2.1  儀器    6
              2.2.2  試劑    6
              2.3 實驗方法    6
              2.3.1  色譜條件    6
              2.3.2  流動相及溶劑的配制    7
              2.3.3  苯甲酸鈉標準品儲備液的配制    7
              2.3.4  可樂、雪碧樣品的處理    7
              2.3.5  線性關系,精密度,重復性,加標回收率研究實驗    7
              2.4結果與討論    8 高效液相色譜和毛細管電泳食品中苯甲酸鈉含量的測定:http://www.kj9998.cn/shiping/20190720/36038.html
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