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              高脂日糧對小鼠子宮發育的影響研究

              時間:2019-07-20 19:50來源:畢業論文
              高脂組小鼠日增重顯著高于對照組,p <0.05,高脂組小鼠成年體重顯著高于對照組,p < 0.05;高脂組小鼠肝臟切片呈現脂肪肝癥狀,且肝臟指標檢測中,總膽固醇(TC)、甘油三酯(TG)

              摘要:高脂日糧能夠誘導小鼠肥胖癥,引起動物肝臟脂肪化以及機體代謝的紊亂,并引起機體內的氧化應激等各類危害,而有關高脂日糧對雌性動物子宮等生殖器官的發育影響以及相關機制的研究目前較少。因此,更深入的研究高能飼料對雌性小鼠子宮發育的影響,不僅對深入研究繁殖系統發育的調節機制具有重要意義,還可以為解決由肥胖引起的各種問題提供新的方法和思路。本研究將10 只雌性 ICR 小鼠隨機平分 2 組,通過長期飼喂實驗組(T)小鼠高脂日糧,對照組(C)普通日糧來誘導肥胖小鼠模型,并應用 HE 染色技術從組織和形態上觀察高脂組的肝臟、子宮及腺體等組織切片與對照組的差異,然后進一步通過免疫組織化學分析來探究高脂日糧對小鼠子宮發育影響的相關蛋白,包括 PARP-1、PARG、caspase-3。結果顯示,高脂組小鼠日增重顯著高于對照組,p <0.05,高脂組小鼠成年體重顯著高于對照組,p < 0.05;高脂組小鼠肝臟切片呈現脂肪肝癥狀,且肝臟指標檢測中,總膽固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)含量均顯著高于對照組,p < 0.05;觀察高脂組小鼠子宮切片發現,子宮腺體比對照組更稀疏,腺體內空腔較窄,形狀為不規則橢圓形,出現部分無腺體區域,而且高脂組小鼠子宮切片上腺體的數目顯著少于對照組,p <0.05;另外,PARP-1、PARG、caspase-3 三種相關蛋白在小鼠子宮內都有不同程度的表達,但各種蛋白在高脂組與對照組上的表達差異不明顯,有待進一步的定量研究。37260
              畢業論文關鍵詞:高脂日糧;肥胖;子宮;腺體;相關蛋白

              源`自^六;維'論.文;網www.kj9998.cn


              Effects of high - fat diet on uterine development of miceAbstract: High fat diet can induce mouse obesity, causing animal liver fat and the body's metabolicdisorders, and cause the body's oxidative stress as well as other hazards, but effects of the high-fat diet onthe female animal uterus and other reproductive organs development as well as their related mechanismsare less studied currently. Therefore, the further study of the effects of high energy feed on uterinedevelopment in female mice is of great significance not only to study the mechanism of reproductivesystem development, but also to provide new methods and ideas for solving various problems caused byobesity. In this study, 10 female ICR mice were randomly pided into two groups. The obese mice modelwas induced by long-term feeding of experimental group (T) mice with high fat diet, the control group (C)was fed with regular diet. The effects of high-fat diet on the uterus development of mice were investigatedby hematoxylin and eosin (H&E) Staining and immunohistochemical (IHC) analysis, including somerelated protein such as PARP-1, PARG and Caspase-3. The results showed that the daily gain of thehigh-fat group was significantly higher than that of the control group, p < 0.05; the adult body weight of thehigh fat group was significantly higher than that of the control group, p < 0.05; the liver slices of the highfat group showed fatty liver symptoms. The levels of total Cholesterol (TC), triglyceride (TG) and lowdensity lipoprotein (LDL-C) were significantly higher than those in the control group (p < 0.05). What’smore, uterine glands are more sparse than the control group, the gland cavity is narrow, the shape ofirregular oval, there part of the absence of glandular area, and high fat group uterine slice of the number ofglands is significantly less than the control group, p < 0.05. In addition, PARP-1, PARG and caspase-3 wereexpressed in different degrees in the mouse uterus, but the expression of PARP-1, PARG and caspase-3 wasnot obvious in the high-fat group and the control group but needs further quantitative studies. 源`自^六;維'論.文;網www.kj9998.cn
              Key words: high-fat diet; obesity; uterus; glands; related proteins
              目 錄
              摘要1
              關鍵詞1 高脂日糧對小鼠子宮發育的影響研究:http://www.kj9998.cn/yixue/20190720/35986.html
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